2011-01-20
An external matrix-assisted laser desorption ionization source for flexible FT-ICR mass spectrometry imaging with internal calibration on adjacent samples
Publication
Publication
J. Am. Soc. Mass Spectrom. , Volume 22 - Issue 1 p. 130- 137
We describe the construction and application of a new MALDI source for FT-ICR mass spectrometry imaging. The source includes a translational X-Y positioning stage with a 10 × 10 cm range of motion for analysis of large sample areas, a quadrupole for mass selection, and an external octopole ion trap with electrodes for the application of an axial potential gradient for controlled ion ejection. An off-line LC MALDI MS/MS run demonstrates the utility of the new source for data- and position-dependent experiments. A FT-ICR MS imaging experiment of a coronal rat brain section yields ∼200 unique peaks from m/z 400–1100 with corresponding mass-selected images. Mass spectra from every pixel are internally calibrated with respect to polymer calibrants collected from an adjacent slide.
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| doi.org/10.1007/s13361-010-0003-x | |
| J. Am. Soc. Mass Spectrom. | |
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Smith, D., Aizikov, K., Duursma, M. C., Giskes, F., Spaanderman, D.-J., McDonnell, L. A., … Heeren, R. (2011). An external matrix-assisted laser desorption ionization source for flexible FT-ICR mass spectrometry imaging with internal calibration on adjacent samples. J. Am. Soc. Mass Spectrom., 22(1), 130–137. doi:10.1007/s13361-010-0003-x |
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